Virtual Gel Analysis

Phytoplasma Resource Center

click here to view virtual gel RFLP patterns iPhyclassifier and virtual gel analysis

(Update to groups coming soon - June14, 2024) This is an example of virtual RFLP patterns of a 16S rDNA nucleotide sequence that was “digested”in silico with 17 restriction endonuclease enzymes. The nucleotide sequence of this 16S rDNA (from cactus witches’-broom phytoplasma) was obtained from the GenBank database (GenBank accession number EU099547). This phytoplasma was originally classified, in 16S rDNA RFLP group 16SrII, on the basis of RFLP patterns that were obtained after actual, wet lab enzymatic digestion of 16S rDNA amplified in the polymerase chain reaction (PCR); classification based on the virtual gel patterns shown here is in complete agreement with the original, wet lab-based classification.