AlfalfaTFDB was generated in the Nemchinov laboratory by a genome-wide profiling using the allele-aware chromosome-level alfalfa genome assembly downloaded from https://figshare.com/projects/whole_genome_sequencing_and_assembly_of_Medicago_sativa/66380 The putative encoded protein sequences were submitted to PlantTFcat (https://plantgrn.noble.org/PlantTFcat/) and PlantTFDB (https://planttfdb.gao-lab.org/prediction_result.php) webservers and transcription factor domains were identified using InterProScan v. 5.44-79.0 (Jones et al. 2014) and ARS-SCINet (https://scinet.usda.gov). The results were consolidated and filtered for putative predicted transcription factors and agreement between prediction programs. For proteins with multiple domains, only the top domain was extracted for each sequence.